留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

微生物参与下高氟区沉积物中氟的迁移行为

张玉贤 甘义群 周肖瑜 姜翠杰 高旭波 李成城

张玉贤, 甘义群, 周肖瑜, 姜翠杰, 高旭波, 李成城. 微生物参与下高氟区沉积物中氟的迁移行为[J]. 地质科技通报, 2022, 41(3): 228-235. doi: 10.19509/j.cnki.dzkq.2022.0078
引用本文: 张玉贤, 甘义群, 周肖瑜, 姜翠杰, 高旭波, 李成城. 微生物参与下高氟区沉积物中氟的迁移行为[J]. 地质科技通报, 2022, 41(3): 228-235. doi: 10.19509/j.cnki.dzkq.2022.0078
Zhang Yuxian, Gan Yiqun, Zhou Xiaoyu, Jiang Cuijie, Gao Xubo, Li Chengcheng. Mobilization of fluoride in sediments at high fluoride area enhanced by microorganisms[J]. Bulletin of Geological Science and Technology, 2022, 41(3): 228-235. doi: 10.19509/j.cnki.dzkq.2022.0078
Citation: Zhang Yuxian, Gan Yiqun, Zhou Xiaoyu, Jiang Cuijie, Gao Xubo, Li Chengcheng. Mobilization of fluoride in sediments at high fluoride area enhanced by microorganisms[J]. Bulletin of Geological Science and Technology, 2022, 41(3): 228-235. doi: 10.19509/j.cnki.dzkq.2022.0078

微生物参与下高氟区沉积物中氟的迁移行为

doi: 10.19509/j.cnki.dzkq.2022.0078
基金项目: 

中国地质调查局地调项目 1212011220949

详细信息
    作者简介:

    张玉贤(1986—), 女, 编辑, 主要从事自然环境研究与高校文化教育方面的工作。E-mail: 308527227@qq.com

    通讯作者:

    周肖瑜(1991—), 女, 工程师, 主要从事土壤与地下水环境相关的科研工作。E-mail: 1335168873@qq.comm

  • 中图分类号: X172

Mobilization of fluoride in sediments at high fluoride area enhanced by microorganisms

  • 摘要:

    氟是自然界广泛分布的非金属元素,也是人体必须的微量元素之一。环境微生物作为自然界中物质迁移的主动力之一,积极地参与着多种元素的水文生物地球化学循环。研究微生物参与下沉积物中氟的释出行为,对深入理解环境氟迁移行为具有重要意义。以山西运城高氟区沉积物样品为代表,采用野外沉积物调查采集、室内环境地球化学分析和微宇宙培养实验等研究手段,从环境生物地球化学作用的角度探讨了微生物介导下沉积物中氟的迁移释出行为。研究证明,高氟区沉积物总氟质量分数介于206.2~781.0 mg/kg之间,主要含氟矿物为长石、云母、方解石、绿泥石、角闪石等。培养条件下,溶液中的氟质量浓度与微生物生长曲线呈现良好的一致性;在培养初期,溶液中的氟质量浓度迅速上升,而后逐渐缓和,14 d后呈下降趋势。其中,黏土类沉积物的释氟量较大,砂土类沉积物释氟量较小。有、无碳源对照培养说明,沉积物中一定量的碳源可被微生物代谢利用,但碳源不是影响微生物在此环境中作用的主要因素。研究证明,原生微生物代谢活动可以显著促进沉积物中氟的迁移释出,微生物作用下不同岩性沉积物中氟的释出特征也有显著的差异,本研究工作有助于进一步丰富环境氟循环的理论认识。

     

  • 图 1  沉积物样品取样点位置示意图

    Figure 1.  Sampling site of sediment samples

    图 2  微生物与氟作用周期培养实验设置图

    Figure 2.  Diagram of microbial and fluorine interaction culture experiment

    图 3  微生物影响氟迁移的验证实验设置图

    1.灭菌处理; 2.灭菌接种菌液; 3.不灭菌; 4.不灭菌+氯霉素(X); 5.不灭菌+放线菌酮(Y); 6.不灭菌+X+Y

    Figure 3.  Setting diagram for the verification of that microorganisms affecting fluorine mobilization

    图 4  培养液取样梯度稀释示意图

    Figure 4.  Schematic diagram of gradient dilution of culture medium sampling

    图 5  微生物作用周期实验培养液中氟质量浓度变化曲线

    Figure 5.  Variation of fluorine concentration in the culture medium during the culture experiment

    图 6  微生物影响氟迁移验证实验中培养液微生物与氟质量浓度变化曲线

    1.沉积物灭菌培养;2.沉积物灭菌后接种土壤菌液培养;3.沉积物不灭菌直接加以培养;4.沉积物不灭菌,再加入氯霉素(X)加以培养;5.沉积物不灭菌,加入放线菌酮(Y)加以培养;6.沉积物不灭菌,加入氯霉素(X)和放线菌酮(Y)加以培养

    Figure 6.  Variation curves of microorganisms and fluorine concentration in the culture medium during the culture experiment

    表  1  普通液体培养基成分表

    Table  1.   Ingredients of common liquid medium

    培养基成分 质量浓度/(g·L-1) 培养基成分 质量浓度或浓度
    (NH4)2SO4 0.24 CoCl2·6H2O 0.17 mg/L
    MgSO4·7H2O 0.12 CuCl2·2H2O 0.10 mg/L
    CaCl2·2H2O 0.20 MnCl2·4H2O 0.10 mg/L
    NaCl 0.10 ZnCl2 0.22 mg/L
    KH2PO4 0.05 酵母提取物 0.50 g/L
    K2HPO4 0.05 乳酸钠 20 mmol/L
    H3BO3 0.000 6 (pH≈7.0)
    下载: 导出CSV

    表  2  沉积物样品基本物化特征统计表

    Table  2.   Basic physical and chemical characteristics of sediment samples

    编号 岩性 深度/m 总氟wB/(mg·kg-1) pH 含水率/%
    QJ-W-02 粉砂 9.2~9.4 292.6 8.47 11.64
    QJ-W-04 细砂 18.4~18.5 373.2 9.13 12.08
    QJ-W-06 砂质黏土 25.3~25.5 781.0 9.96 21.41
    QJ-W-10 粉质黏土 32.0~32.2 618.2 9.48 23.59
    QJ-W-13 细砂 41.8~42.0 206.2 9.58 8.42
    下载: 导出CSV

    表  3  沉积物培养液中特征指标Pearson相关系数表

    Table  3.   Pearson correlation coefficient of characteristic indexes in sediment culture medium

    项目 编号 ρ(F)-pH ρ(F)-BM pH-BM
    沉积物未灭菌 02-3 0.750 0.714 0.522
    04-3 0.774* 0.477 0.666
    06-3 0.593 0.659 0.490
    10-3 0.651 0.528 0.424
    13-3 0.101 0.543 0.655
    全部 0.477* 0.469* 0.551*
    沉积物灭菌接种土壤菌液 02-2 0.509 -0.258 -0.516
    04-3 0.233 0.460 -0.402
    06-2 0.090 -0.764* -0.456
    10-2 0.237 0.134 -0.133
    13-2 0.658 0.276 -0.263
    全部 0.129 -0.015 -0.289
    注:BM代表微生物数量;*表示置信区间在0.05水平(双侧)上显著相关
    下载: 导出CSV
  • [1] Anderson J A, Antich P P, Prior J O. Stimulated positron emission analysis techniques for the quantitative assessment of fluorine in bone[J]. IEEE Transactions on Nuclear Science, 1991, 38(2): 713-718. doi: 10.1109/23.289379
    [2] Gao X B, Wang Y X, Li Y L, et al. Enrichment of fluoride in groundwater under the impact of saline water intrusion at the salt lake area of Yuncheng Basin, northern China[J]. Environmental Geology, 2007, 53: 795-803. doi: 10.1007/s00254-007-0692-z
    [3] 王滨滨, 郑宝山, 廖昂. 氟在土壤中的富集与淋滤[J]. 矿物学报, 2010, 30(4): 496-500. https://www.cnki.com.cn/Article/CJFDTOTAL-KWXB201004016.htm

    Wang B B, Zheng B S, Liao A. Fluoride enrichment and leaching in the soil: A review[J]. Acta Mineralogica Sinica, 2010, 30(4): 496-500(in Chinese with English abstract). https://www.cnki.com.cn/Article/CJFDTOTAL-KWXB201004016.htm
    [4] Li C C, Gao X B, Wang Y X. Hydrogeochemistry of high-fluoride groundwater at Yuncheng Basin, northern China[J]. Science of the Total Environment, 2015, 508: 155-165. doi: 10.1016/j.scitotenv.2014.11.045
    [5] 任福弘, 曾溅辉, 刘文生, 等. 高氟地下水的水文化学环境及氟的赋存形式与地氟病患病率的关系: 以华北平原为例[J]. 地球学报, 1996, 17(1): 85-96. https://www.cnki.com.cn/Article/CJFDTOTAL-DQXB601.008.htm

    Ren H F, Zeng J H, Liu W S, et al. Hydrogeochemical environment of high fluorine groundwater and the relation between the speciation of fluorine and the diseased ratio of endemic fluorosis: A case study of the North China Plain[J]. Acta Geoscientica Sinica, 1996, 17(1): 85-96(in Chinese with English abstract). https://www.cnki.com.cn/Article/CJFDTOTAL-DQXB601.008.htm
    [6] 刘东生, 陈庆沐, 余志成, 等. 我国地方性氟病的地球化学问题[J]. 地球化学, 1980, 9(1): 13-22. doi: 10.3321/j.issn:0379-1726.1980.01.002

    Liu D S, Chen Q M, Yu Z C, et al. Geochemical environment problems concerning the endemic fluorine disease in China[J]. Geochimica, 1980, 9(1): 13-22(in Chinese with English abstract). doi: 10.3321/j.issn:0379-1726.1980.01.002
    [7] 江欣悦, 李静, 郭林, 等. 豫北平原浅层地下水化学特征与成因机制[J]. 地质科技通报, 2021, 40(5): 290-300. doi: 10.19509/j.cnki.dzkq.2021.0511

    Jiang X Y, Li J, Guo L, et al. Chemical characteristics and formation mechanism of shallow groundwater in the northern Henan Plain[J]. Bulletin of Geological Science and Technology, 2021, 40(5): 290-300(in Chinese with English abstract). doi: 10.19509/j.cnki.dzkq.2021.0511
    [8] Huang P M, Wang M K. Soil mineral-organic matter-microbe interactions: Impacts on biogeochemical processes and biodiversity in soils[J]. Pedobiologia, 2005, 49(6): 609-635. doi: 10.1016/j.pedobi.2005.06.006
    [9] 刘金辉, 吴为荣, 刘亚洁, 等. 浸铀过程中氧化硫硫杆菌的耐氟性试验研究[J]. 金属矿山, 2009, 39(5): 52-52, 66. doi: 10.3321/j.issn:1001-1250.2009.05.013

    Liu J H, Wu W R, Liu Y J, et al. Study on the fluorine resistance of thiobacillus thiooxidans in uranium leaching[J]. Metal Mine, 2009, 39(5): 50-52, 66(in Chinese with English abstract). doi: 10.3321/j.issn:1001-1250.2009.05.013
    [10] Zhang X, Gao X B, Li C C, et al. Fluoride contributes to the shaping the microbial community in high fluoride groundwater in Qiji County, Yuncheng City, China[J]. Scientific Report, 2019, 9: 14488. doi: 10.1038/s41598-019-50914-6
    [11] Zhou J P, Wang H M, Charles A C, et al. Dissolution of fluorapatite by pseudomonas fluorescens P35 resulting in fluorine release[J]. Geomicrobiology Journal, 2017, 34(5): 421-433.
    [12] Gao X B, Luo W T, Luo X S, et al. Indigenous microbes induced fluoride release from aquifer sediments[J]. Environmental Pollution, 2019, 252: 580-590. doi: 10.1016/j.envpol.2019.05.118
    [13] Li C C, Gao X B, Liu Y S, et al. Impact of anthropogenic activities on the enrichment of fluoride and salinity in groundwater in the Yuncheng Basin constrained by Cl/Br ratio, δ18O, δ2H, δ13C and δ7 Li isotopes[J]. Journal of Hydrology, 2019, 579: 124211. doi: 10.1016/j.jhydrol.2019.124211
    [14] Li D N, Gao X B, Wang Y X. et al. Diverse mechanisms drive fluoride enrichment in groundwater in two neighboring sites in northern China[J]. Environmental Pollution, 2018, 237: 430-441. doi: 10.1016/j.envpol.2018.02.072
    [15] Areco M M, Hanela S, Duran J, et al. Biosorption of Cu(Ⅱ), Zn(Ⅱ), Cd(Ⅱ) and Pb(Ⅱ) by dead biomasses of green alga Ulva lactuca and the development of a sustainable matrix for adsorption implementation[J]. Journal of Hazardous Materials, 2012, 213: 123-132.
    [16] Dikshit R, Jain A, Dey A, et al. Microbially induced calcite precipitation using Bacillus velezensis with guar gum[J]. PloS One, 2020, 15(8): e0236745. doi: 10.1371/journal.pone.0236745
    [17] 宋新华. 氟在花岗岩类岩石中的性状[J]. 地质科技情报, 1985, 4(4): 28-35. https://www.cnki.com.cn/Article/CJFDTOTAL-DZKQ198504008.htm

    Song X H. Properties of fluorine in granitoid rocks[J]. Geological Science and Technology Information, 1985, 4(4): 28-35(in Chinese with English abstract). https://www.cnki.com.cn/Article/CJFDTOTAL-DZKQ198504008.htm
    [18] Harrington L F, Cooper E M, Vasudevan D. Fluoride sorption and associated aluminum release in variable charge soils[J]. Journal of Colloid and Interface Science, 2003, 267(2): 302-313. doi: 10.1016/S0021-9797(03)00609-X
    [19] Luo W T, Gao X B, Zhang X. Geochemical processes controlling the groundwater chemistry and fluoride contamination in the Yuncheng Basin, China: An area with complex hydrogeochemical conditions[J]. PloS One, 2018, 13(7): e0199082. doi: 10.1371/journal.pone.0199082
    [20] 潘欢迎, 邹常健, 毕俊擘, 等. 新疆阿克苏典型山前洪积扇内高氟地下水的化学特征及氟富集机制[J]. 地质科技通报, 2021, 40(3): 194-203. doi: 10.19509/j.cnki.dzkq.2021.0312

    Pan H Y, Zou C J, Bi J B, et al. Hydrochemical characteristics and fluoride enrichment mechanisms of high-fluoride groundwater in a typical piedmont proluvial fan in Aksu area, Xinjiang, China[J]. Bulletin of Geological Science and Technology, 2021, 40(3): 194-203(in Chinese with English abstract). doi: 10.19509/j.cnki.dzkq.2021.0312
    [21] 余浩文, 刘昭, 荣峰, 等. 西藏错那地热田水化学特征与物源机制[J]. 地质科技通报, 2021, 40(3): 34-44. doi: 10.19509/j.cnki.dzkq.2021.0318

    Yu H W, Liu Z, Rong F, et al. Characteristics and source mechanism of geothermal field in Cuona, Tibet[J]. Bulletin of Geological Science and Technology, 2021, 40(3): 34-44(in Chinese with English abstract). doi: 10.19509/j.cnki.dzkq.2021.0318
  • 加载中
图(6) / 表(3)
计量
  • 文章访问数:  33
  • HTML全文浏览量:  16
  • PDF下载量:  8
  • 被引次数: 0
出版历程
  • 收稿日期:  2021-07-26

目录

    /

    返回文章
    返回