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微生物参与下高氟区沉积物中氟的迁移行为

张玉贤 甘义群 周肖瑜 姜翠杰 高旭波 李成城

张玉贤, 甘义群, 周肖瑜, 姜翠杰, 高旭波, 李成城. 微生物参与下高氟区沉积物中氟的迁移行为[J]. 地质科技通报, 2022, 41(3): 228-235. doi: 10.19509/j.cnki.dzkq.2022.0078
引用本文: 张玉贤, 甘义群, 周肖瑜, 姜翠杰, 高旭波, 李成城. 微生物参与下高氟区沉积物中氟的迁移行为[J]. 地质科技通报, 2022, 41(3): 228-235. doi: 10.19509/j.cnki.dzkq.2022.0078
Zhang Yuxian, Gan Yiqun, Zhou Xiaoyu, Jiang Cuijie, Gao Xubo, Li Chengcheng. Mobilization of fluoride in sediments at high fluoride area enhanced by microorganisms[J]. Bulletin of Geological Science and Technology, 2022, 41(3): 228-235. doi: 10.19509/j.cnki.dzkq.2022.0078
Citation: Zhang Yuxian, Gan Yiqun, Zhou Xiaoyu, Jiang Cuijie, Gao Xubo, Li Chengcheng. Mobilization of fluoride in sediments at high fluoride area enhanced by microorganisms[J]. Bulletin of Geological Science and Technology, 2022, 41(3): 228-235. doi: 10.19509/j.cnki.dzkq.2022.0078

微生物参与下高氟区沉积物中氟的迁移行为

doi: 10.19509/j.cnki.dzkq.2022.0078
基金项目: 

中国地质调查局地调项目 1212011220949

详细信息
    作者简介:

    张玉贤(1986—), 女, 编辑, 主要从事自然环境研究与高校文化教育方面的工作。E-mail: 308527227@qq.com

    通讯作者:

    周肖瑜(1991—), 女, 工程师, 主要从事土壤与地下水环境相关的科研工作。E-mail: 1335168873@qq.comm

  • 中图分类号: X172

Mobilization of fluoride in sediments at high fluoride area enhanced by microorganisms

  • 摘要:

    氟是自然界广泛分布的非金属元素,也是人体必须的微量元素之一。环境微生物作为自然界中物质迁移的主动力之一,积极地参与着多种元素的水文生物地球化学循环。研究微生物参与下沉积物中氟的释出行为,对深入理解环境氟迁移行为具有重要意义。以山西运城高氟区沉积物样品为代表,采用野外沉积物调查采集、室内环境地球化学分析和微宇宙培养实验等研究手段,从环境生物地球化学作用的角度探讨了微生物介导下沉积物中氟的迁移释出行为。研究证明,高氟区沉积物总氟质量分数介于206.2~781.0 mg/kg之间,主要含氟矿物为长石、云母、方解石、绿泥石、角闪石等。培养条件下,溶液中的氟质量浓度与微生物生长曲线呈现良好的一致性;在培养初期,溶液中的氟质量浓度迅速上升,而后逐渐缓和,14 d后呈下降趋势。其中,黏土类沉积物的释氟量较大,砂土类沉积物释氟量较小。有、无碳源对照培养说明,沉积物中一定量的碳源可被微生物代谢利用,但碳源不是影响微生物在此环境中作用的主要因素。研究证明,原生微生物代谢活动可以显著促进沉积物中氟的迁移释出,微生物作用下不同岩性沉积物中氟的释出特征也有显著的差异,本研究工作有助于进一步丰富环境氟循环的理论认识。

     

  • 图 1  沉积物样品取样点位置示意图

    Figure 1.  Sampling site of sediment samples

    图 2  微生物与氟作用周期培养实验设置图

    Figure 2.  Diagram of microbial and fluorine interaction culture experiment

    图 3  微生物影响氟迁移的验证实验设置图

    1.灭菌处理; 2.灭菌接种菌液; 3.不灭菌; 4.不灭菌+氯霉素(X); 5.不灭菌+放线菌酮(Y); 6.不灭菌+X+Y

    Figure 3.  Setting diagram for the verification of that microorganisms affecting fluorine mobilization

    图 4  培养液取样梯度稀释示意图

    Figure 4.  Schematic diagram of gradient dilution of culture medium sampling

    图 5  微生物作用周期实验培养液中氟质量浓度变化曲线

    Figure 5.  Variation of fluorine concentration in the culture medium during the culture experiment

    图 6  微生物影响氟迁移验证实验中培养液微生物与氟质量浓度变化曲线

    1.沉积物灭菌培养;2.沉积物灭菌后接种土壤菌液培养;3.沉积物不灭菌直接加以培养;4.沉积物不灭菌,再加入氯霉素(X)加以培养;5.沉积物不灭菌,加入放线菌酮(Y)加以培养;6.沉积物不灭菌,加入氯霉素(X)和放线菌酮(Y)加以培养

    Figure 6.  Variation curves of microorganisms and fluorine concentration in the culture medium during the culture experiment

    表  1  普通液体培养基成分表

    Table  1.   Ingredients of common liquid medium

    培养基成分 质量浓度/(g·L-1) 培养基成分 质量浓度或浓度
    (NH4)2SO4 0.24 CoCl2·6H2O 0.17 mg/L
    MgSO4·7H2O 0.12 CuCl2·2H2O 0.10 mg/L
    CaCl2·2H2O 0.20 MnCl2·4H2O 0.10 mg/L
    NaCl 0.10 ZnCl2 0.22 mg/L
    KH2PO4 0.05 酵母提取物 0.50 g/L
    K2HPO4 0.05 乳酸钠 20 mmol/L
    H3BO3 0.000 6 (pH≈7.0)
    下载: 导出CSV

    表  2  沉积物样品基本物化特征统计表

    Table  2.   Basic physical and chemical characteristics of sediment samples

    编号 岩性 深度/m 总氟wB/(mg·kg-1) pH 含水率/%
    QJ-W-02 粉砂 9.2~9.4 292.6 8.47 11.64
    QJ-W-04 细砂 18.4~18.5 373.2 9.13 12.08
    QJ-W-06 砂质黏土 25.3~25.5 781.0 9.96 21.41
    QJ-W-10 粉质黏土 32.0~32.2 618.2 9.48 23.59
    QJ-W-13 细砂 41.8~42.0 206.2 9.58 8.42
    下载: 导出CSV

    表  3  沉积物培养液中特征指标Pearson相关系数表

    Table  3.   Pearson correlation coefficient of characteristic indexes in sediment culture medium

    项目 编号 ρ(F)-pH ρ(F)-BM pH-BM
    沉积物未灭菌 02-3 0.750 0.714 0.522
    04-3 0.774* 0.477 0.666
    06-3 0.593 0.659 0.490
    10-3 0.651 0.528 0.424
    13-3 0.101 0.543 0.655
    全部 0.477* 0.469* 0.551*
    沉积物灭菌接种土壤菌液 02-2 0.509 -0.258 -0.516
    04-3 0.233 0.460 -0.402
    06-2 0.090 -0.764* -0.456
    10-2 0.237 0.134 -0.133
    13-2 0.658 0.276 -0.263
    全部 0.129 -0.015 -0.289
    注:BM代表微生物数量;*表示置信区间在0.05水平(双侧)上显著相关
    下载: 导出CSV
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  • 收稿日期:  2021-07-26

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