Abstract: This paper analyzes the features of hydrogen and oxygen isotopes ²H,¹⁸O and ³H in geothermal water from hot spring and geothermal well based on sampling and testing.The study identifies the makeup source of geothermal water in east Shandong Province by isotope geochemistry method.Combining the elevation effect,the paper selects the area with relatively high hills as the area to be made-up.In addition,the paper uses ³H testing data and the result of J.FT.experience-based estimation and constructs the relation between δ¹⁸O and latitude to determine the make-up source for geothermal water in east Shandong Province-meteoric water.The results show that the make-up of geothermal water is the rainfall.