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Cr(Ⅲ)对锰氧化菌P.putida MnB1活性及功能的影响规律与机制

尹元雪 赵雨溪 孙群群 童曼

尹元雪, 赵雨溪, 孙群群, 童曼. Cr(Ⅲ)对锰氧化菌P.putida MnB1活性及功能的影响规律与机制[J]. 地质科技通报, 2024, 43(1): 298-305. doi: 10.19509/j.cnki.dzkq.tb20220351
引用本文: 尹元雪, 赵雨溪, 孙群群, 童曼. Cr(Ⅲ)对锰氧化菌P.putida MnB1活性及功能的影响规律与机制[J]. 地质科技通报, 2024, 43(1): 298-305. doi: 10.19509/j.cnki.dzkq.tb20220351
YIN Yuanxue, ZHAO Yuxi, SUN Qunqun, TONG Man. Effect of Cr(Ⅲ) on the activity and function of Mn(Ⅱ)-oxidizing bacteria Pseudomonas putida MnB1[J]. Bulletin of Geological Science and Technology, 2024, 43(1): 298-305. doi: 10.19509/j.cnki.dzkq.tb20220351
Citation: YIN Yuanxue, ZHAO Yuxi, SUN Qunqun, TONG Man. Effect of Cr(Ⅲ) on the activity and function of Mn(Ⅱ)-oxidizing bacteria Pseudomonas putida MnB1[J]. Bulletin of Geological Science and Technology, 2024, 43(1): 298-305. doi: 10.19509/j.cnki.dzkq.tb20220351

Cr(Ⅲ)对锰氧化菌P.putida MnB1活性及功能的影响规律与机制

doi: 10.19509/j.cnki.dzkq.tb20220351
基金项目: 

国家自然科学基金青年科学基金项目 41703113

详细信息
    作者简介:

    尹元雪, E-mail: 1269769379@qq.com

    通讯作者:

    童曼, E-mail: Tongman@cug.edu.cn

  • 中图分类号: X172

Effect of Cr(Ⅲ) on the activity and function of Mn(Ⅱ)-oxidizing bacteria Pseudomonas putida MnB1

More Information
  • 摘要:

    三价铬(Cr(Ⅲ))与锰氧化菌在环境中通常伴同存在,锰氧化菌介导生成的锰氧化物是Cr(Ⅲ)的主要天然氧化剂,探究Cr(Ⅲ)对锰氧化菌活性和功能的影响对于阐明环境中的锰循环和铬的迁移转化行为具有重要意义。以锰氧化模式菌Pseudomonas putida MnB1为研究对象,通过批实验探究了Cr(Ⅲ)对MnB1活性与功能的影响及其机制。结果表明,Cr(Ⅲ)浓度高于0.05 mmol/L时会造成MnB1的显著死亡,且Cr(Ⅲ)浓度越高杀菌作用越显著,当Cr(Ⅲ)高于0.02 mmol/L时会推迟锰的生物氧化,当Cr(Ⅲ)浓度高于0.2 mmol/L时完全抑制锰的生物氧化。机制研究表明,Cr(Ⅲ)诱导产生的胞内活性氧和Cr(Ⅲ)颗粒引起的细胞膜通透性改变共同影响了MnB1的生长代谢和功能。研究结果为认识锰的生物地球化学循环和铬的迁移转化行为提供了新的理论依据。

     

  • 图 1  Cr(Ⅲ)对锰氧化菌MnB1活性的影响(N表示稀释涂布平板测定的活菌数量)

    Figure 1.  Effect of Cr(Ⅲ) on the activity of Mn-oxidizing bacteria MnB1(N represents the number of live bacteria measured by dilution coating plate)

    图 2  Cr(Ⅲ)对锰氧化菌MnB1功能的影响

    Figure 2.  Effect of Cr(Ⅲ) on the function of Mn-oxidizing bacteria MnB1

    图 3  铬形态的变化

    Figure 3.  Transformations of Cr species

    图 4  胞内ROS荧光强度

    Figure 4.  Fluorescence intensity of intracellular ROS produced with the addition of Cr(Ⅲ)

    图 5  Cr(Ⅲ)对MnB1细胞通透性的影响

    Figure 5.  Effect of Cr(Ⅲ) on the permeability of MnB1 cells

    图 6  Cr(Ⅲ)对MnB1生物膜发育的影响

    Figure 6.  Effect of Cr(Ⅲ) on the development of MnB1 biofilms

    图 7  Cr(Ⅲ)对EPS分泌的影响

    Figure 7.  Effect of Cr(Ⅲ) on the secretion of EPS

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出版历程
  • 收稿日期:  2022-07-14
  • 录用日期:  2023-02-07
  • 修回日期:  2023-01-11

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